Antibiogram pattern of bacteria causing endometritis in cows

Aim : To find out the organisms causing endometritis in cattle and to determine their in vitro sensitivity to various antibiotics Materials and methods: Thirty uterine secretion samples, 9 from Holstein Friesian and 21 from Jersey cows were collected in and around Namakkal district of Tamil Nadu from clinical cases of endometritis. The bacteria isolated both aerobically and anaerobically from endometritis showed the characteristic colony, were gram stained and confirmed by standard biochemical tests. The invitro antibiotic sensitivity test with different antibiotic discs were carried out. Results and Discussion: Out of 30 samples, 25 contained different strains of bacteria and 5 were found bacteriologically sterile. Different species of bacteria isolated from these samples were, Escherichia coli (36.66%), Klebsiella spp (30%), Proteus spp (13.33%), Pseudomonas aeruginosa (6.66%), Clostridium spp (3.33%). The in vitro antibiotic sensitivity test indicated that the highest number of isolates (64%) were sensitive to Ceftriaxone, followed by Gentamicin, Enrofloxacin and Chlortetracycline (32%). Chloramphenicol showed sensitivity in minimum number (8%) of isolates. Conclusion : This study revealed that the bacteria E.coli and Klebisella spp are more commonly isolated in endometritis in cows and the drug Ceftriaxone is highly effective.